دکتر مریم شهیدی

Education & Training :

• Nov.2007 - Dec.2007
  • ART Laboratory and Medical Embryology Education,
  • Department of Gynaecology and Obstetrics,Reproductive Medicine Unit, Campus of Luebeck, Germany
• Mar. 2004 – Sep. 2004
  • Research visitor
  • Department of Radiation Biology, University Hospital, Essen, Germany
• Sep.1998 – Nov. 2004
  • Ph.D. Student, Medical Physics
  • Tarbiat Modarres University, Tehran, Iran
• Sep. 1992 – Jul.1996
  • M.Sc. Student, Medical Physics
  • Tarbiat Modarres University, Tehran, Iran
• Sep.1990 – Jul.1992
  • B.Sc. Student, English translation,
  • Azad University, (unfinished)
• Feb.1984 – Jul.1988
  • B.Sc. Student, Applied Physics (Nuclear Physics)
  • Shahid Beheshti University, Tehran, Iran

PROFESSIONAL EXPERIENCE :

• Sep.1996 – Present
  • Official Member of Scientific Board
  • Medical Faculty, Mazandaran University of Medical Sciences,Sari,Iran.
  • [Assistant professor (2004 - present) – Educator and Researcher (1996 -2004)]
  • Department Of Biochemistry, Biophysics and Genetics Mazandaran University of Medical Sciences, School of Medicine, Sari, Iran
• Dec. 2004 – Present
  • Embryologist and Director
  • Hazrate Maryam Fertility Center, Nimeh Shaban Hospital Sari, Iran
• Sep.1990 – Sep.1992
  • Teacher of physics
  • Ministry of Education, Sari, Iran

SPECIAL SKILLS :

• In vivo Micronucleus assay (on bone marrow cells)
• Alkaline and Neutral Comet assay (with whole blood, separated lymphocytes, sperm)
• Fluorescent Insitue Hybridization (FISH) on Sperm, Blastomer , Lymphocyte
• DNA diffusion Assay for Apoptosis Assessment
• Assisted Reproductive Techniques and Laboratory management:
  • Handling and thorough analysis of semen.
  • Sperm preparation techniques for IUI, IVF and ICSI.
  • Methods of surgical sperm recovery for ICSI in cases of azoospermia and the further handling,
  • cryopreservation, thawing and preparation of the retrieved sperm.
  • Preparation of laboratory media and tools.
  • Oocytes collection, identification and denudation.
  • IVF technique.
  • ICSI manipulation.
  • Evaluation and scoring of fertilization and embryo quality.
  • Preparation of embryos for transfer.
  • Gametes and embryo cryopreservation by vitrification.
  • Embryo transfer.
  • Laboratory management and Logistic support
• Blastomer Biopsy

SPECIAL TRAININGS and CERTIFICATES :

• 13-25 June 2011
  • Training and Education Courses and Workshop
  • Advanced Tecnologies Laboratory Reproductive Biology and Technology Paris, France
• 28Feb. - 1 March 2011
  • Workshop on Preimplantation genetic Diagnosis
  • Tarbiat Modares University, Tehran, Iran
• 7-8 Feb 2008
  • Workshop on Application of sperm evaluation techniques in diagnosis and treatment of infertility
  • Rooyan Fertility and Research Institute , Esfehan, Iran
• 9 March 2006
  • Workshop on Sperm Evaluation Techniques
  • Ave Sina Fertility and Research Institute, Tehran, Iran
• 1- 2 March 2006
  • Gamete & Embryo Donation in Infertility Treatment
  • Ave Sina Fertility and Research Institute, Tehran, Iran
• 29-31 Jan. 2006
  • Workshop on improved Techniques in Infertility Treatment
  • Rooyan Fertility and Research Institute , Tehran, Iran
• 7-11 Feb. 2003
  • Workshop on Comet Assay: Application in Toxicology and Molecular Epidemiology
  • Lucknow, India

PUBLICATIONS :

• Mesbah Namin S.A., Shahidi M:., Nakhshab M. ,An Increased Genotoxic Risk in lymphocytes of the Phototherapy-treated Hyperbilirubinemic Neonates, has been accepted for publication in the Iranian Biomedical Journal (IBJ).
• Rafat N., Shabestani Monfared A., Shahidi M:., Allahverdi Pourfallah T., The modulating effect of royal jelly consumption against radiation-induced apoptosis in human peripheral blood leukocytes, Journal of Medical Physics, 2016 Jan-Mar; 41(1): 52–57.
• Samadaee Gelehkolaee K., Mccarthy B.M, Khalilian A. , Hamzehgardeshi Z. , Peyvandi S., Elyasi F., Shahidi M:., Factors Associated With Marital Satisfaction in Infertile Couple: A Comprehensive Literature Review, Global Journal of Health Science; 2016 May; 8(5): 96–109.
• Ghasemi A., Pourfallah T.A., Akbari M.R., Babapour H., Shahidi M:.,Photo neutron dose equivalent rate in 15 MV X-ray beam from a Siemens Primus Linac. Journal of Medical Physics. 2015 Apr-Jun; 40(2):90-4.
• Ehsani Sarvkolai H, Shahidi M:, Yaghoubi A, The comparison of depression, anxiety, self-esteem and life satisfaction between fertilized and infertilized women, Trends In Life Sciences, Volume 3, Special Issue1, 2014 Page 97-101.
• Farokhi F; Jamili, Sh; Shahidi M:.; Mashinchian A.; Vosoughi Gh. H, The effect of Malathion on the hematological parameters of Rutilus Rutilus Caspicus, Nautilus Journal, Volume 128, Issue 3 (No:1) December 28, 2014 Page 105-113.
• Farokhi F; Shahidi M:.; Jamili, Sh; Mashinchian A.; Vosoughi Gh. H, Studying The effect of Malathion on the liver and gill tissues of Rutilus Rutilus Caspicus, Journal of Animal Environment, 2013(in persian).
• Pourfallah TA, Babapour H, Shahidi M:, Evaluation of High Level Environmental Background Radiation Areas and its Variation in Ramsar, Iranian Journal of Medical Physics, Vol. 9, No. 2, Spring 2012, Page 87-92.
• Shahidi M:, Mozdarani S, Shammas Sh, Interindividual differences in radiation-induced apoptosis of peripheral blood leukocytes in normal individuals and breast cancer patients, IJRR, (9-4) - 2012 March.
• Zabihi Rigcheshmeh M, Mirzaiian B, Hasanzadeh R, Shahidi M:, A comparative study of sexual point of view in Iranian infertile and fertile spouses. Journal of Mazandaran University of Medical Sciences, 2011, Special Issue Feb.20th, Page 274-283 (in persian).
• Aghamohammadi A, Shahidi M:, Male body mass index and sperm parameters, Gineco, 2011: Anul VII, Vol 7, Nr. 24, 92-93.
• Asghari F, Shahidi M:, Chashnidel Y, Deldar H, Ansari-Pirsaraei Z, Mohammadi-Sangcheshmeh A, 205 glucose-6-phosphate dehydrogenaze activity in ovine oocytes is associated with pre-implantation embryonic development in vitro. Reprod Fertil Dev. 2011; 23(1):201-2.
• Shahidi M:, Mozdarani H, Mueller WU , Radiosensitivity and repair kinetics of gamma-irradiated leukocytes from sporadic prostate cancer patients and healthy individuals assessed by alkaline comet assay. Iran Biomed J. 2010 Jul; 14(3):67-75.
• Babazadeh Z, Razavi Sh, Tavalaee M, Deemeh MR, Shahidi M:, Nasr-Esfahani MH, Sperm DNA damage and its relation with leukocyte DNA damage. Reproductive Toxicology 29 (2010) 120–124.
• Shahidi M:, Mozdarani H, Bryant PE, Radiation sensitivity of leukocytes from healthy individuals and breast cancer patients as measured by the alkaline and neutral comet assay, Cancer Letters, 257 (2007) 263–273
• Shahidi M:, Mozdarani H, Samei F. Studying the radiation sensitivity and repair kinetics of leukocytes from healthy individuals and prostate cancer patients as measured by the alkaline comet assay. Kowsar Medical Journal, fall 2005, Vol 10, Number3, Pages 21-31 (in Persian).
• Shahidi M:, Mozdarani H, Mokhtari dizaji M, Comparison of the radiation sensitivity of leukocytes from healthy individuals and breast cancer patients as measured by the alkaline comet assay. Journal of Mazandaran University of Medical Sciences, 2004, Vol 45 Pages 104-116 (in Persian).
• Shahidi M:, Study of leukocyte radiosensitivity of normal individuals and breast and prostate cancer patients following various doses of gamma rays induced DNA damage using comet assay. (Ph.D. graduation thesis) Autumn 2004.
• Shahidi M:, Mozdarani H, Potent radioprotective effect of therapeutic doses of Ranitidine and Famotidine against gamma rays induced micronuclei in vivo. Iranian Journal of Radiation Research, 2003,1(1):29-35.
• Shahidi M:, Mozdarani H, Samei F, Studying the effects of famotidine on clastogenic effects of gamma rays in mice bone marrow cells by means of micronucleus test. Journal of Mazandaran University of Medical Sciences, 2000, Vol 10, No26, Page19-26 (in Persian).
• Shahidi M:, Mozdarani H, Studying the radioprotective effects of ranitidine against gamma rays. Journal of Mazandaran University of Medical Sciences, 1998, Vol 7-8, No17-18, Page50-55 (in Persian).
• Shahidi M:, Radioprotective effects of Ranitidine and Famotidine in mice bone marrow cells exposed to gamma rays. (M.Sc. graduation thesis) Jul.1996.

Posters and Lectures :

• Rafat N, Shabestani Monfared A, Shahidi M, Studying the radioprotective effects of Royal Gelly on radiation induced DNA damage on leukocytes by means of comet assay, 3rd national Congress on Cellular and Mulecular News n Contagious Diseases.13-15 May 2015, Babol, Iran (Abstract in persian).
• Shahidi M, Karimpour F, Karimi R, Hamzehgardeshi Z. The role of heparin therapy on pregnancy rate in women with in vitro fertilization implantation failure: A randomized controlled trial. Has been accepted for Poster Display at the 21st COGI Congress, taking place May 14-16, 2015, in Frankfurt, Germany.
• Hamzehgardeshi Z, Shahidi M, Karimpour F, Karimi R. Serum concentration of free testestrone and pre-eclampsia: a prospective, nested -case control study. Has been accepted for Poster Display at the 21st COGI Congress, taking place May 14-16, 2015, in Frankfurt, Germany.
• Shirazi MM, Shabestani Monfared A, Shahidi M, Does myocardial scan with Technetium lead to radioadaptive response among patients who are under Thallium scan ? (cytogenetic study), 11th International Congress of Medical Physics, Nov.5-7 2014 Tehran, Iran (Abstract in persian).
• Shahidi M, Mesbah Namin SA, Increased DNA double strand breaks and apoptosis in lymphocytes from phototherapy-treated hyperbilirubinemic neonates, 11th International Congress of Medical Physics, Nov.5-7 2014 Tehran, Iran. (Abstract in persian).
• Shahidi M, Karimpour F, Karimi R, Hamzehgardeshi Z. A randomized controlled trial of heparin therapy for women with in vitro fertilization implantation failure. Iranian Journal of Reproductive Medicine, Vol. 12, No 6 (Suppl. 1), June 2014. (Abstract)
• Karimi R, Galinimoghaddam T, Moslemizadeh N, Shahidi M, Karimpour FZ, Hamzehgardeshi Z. The Relationship between serum free testosterone level and pre-eclampsia. Iranian Journal of Reproductive Medicine, Vol. 12, No 6 (Suppl. 1), June 2014. (Abstract).
•     Rajabifar S, Mozdarani H, Shahidi M, DNA Damage in spermatozoa of Infertile Men in North of Iran. 6th national congress IRHRC reproductive and infertility updates, Tehran-Iran, 8 - 10 Jan 2014.
• Rajabifar S, Mozdarani H, Shahidi M, DNA Damage in Leukocytes of three groups of Infertile Men that’s refer to Hazrate Maryam infertility center in sari, 6th national congress IRHRC reproductive and infertility updates, Tehran-Iran, 8 - 10 Jan 2014.
• Zabihi rigcheshme M, Mirzaian B, Hasanzade R, Shahidi M, The relationship between marital satisfaction and sexual attitude in fertile and infertile couples, 6th national congress IRHRC reproductive and infertility updates, Tehran-Iran, 8 - 10 Jan 2014.
• Shahidi M, Mozdarani H, Shammas Sh, Detection of individual differences in radiation-induced apoptosis of peripheral blood lymphocytes in normal individuals and breast cancer patients using neutral comet assay. The 1st MEFOMP International Conference of Medical Physics, Shiraz-Iran, Nov 2-4, 2011.(Intractive Poster Presentation).
• Zabihi Rigcheshmeh M, Mirzaiian B, Hasanzadeh R, Shahidi M, A comparative study of sexual point of view in Iranian infertile spouses , Fifth Conference on Sexual Health, 27-28 October 2011, Shahed University (Poster).
• Zabihi Rigcheshmeh M, Mirzaiian B, Hasanzadeh R, Shahidi M, A comparative study of sexual attitude in Iranian infertile spouses ,Malaysian Psychology Conference, 22 October2011, Nottingham University’s Malaysia Campus (Poster).
• Asghari F, Shahidi M, Chashnidel Y, Deldar H, Ansari-Pirsaraei Z, Mohammadi-Sangcheshmeh A. 205 glucose-6-phosphate dehydrogenaze activity in ovine oocytes is associated with pre-implantation embryonic development in vitro. The Annual Conference of the International Embryo Transfer Society, Orlando, Florida, USA. 8–12 January 2011 (poster).
• Shahidi M, Peyvandi S, Jorsaraii Gh, Nasri Sh, Zabiyani A,Mohamadi Sangcheshmeh A, Cleavage rate as a predictor of pregnancy success in ICSI cycles, 13 th Word Congress on Controvercies on Obstetrics, Gynecology and Infertility, Berlin, Germany, Nov 4-7, 2010. (Poster).
• Mozdarani S, Shahidi M, Mozdarani H, Repair kinetics of radiation induced DNA damages in leukocytes of breast cancer patients as a measure of breast cancer susceptibility. Presentation - Florence, Italy (14th World Congress of Gynecological Endocrinology) March 1, 2010 (Oral presentation).
• Shahidi M., Mozdarani H. Evaluation Of Radiation Induced DNA Damage And DNA Repair In Prostate And Breast Cancer Patients Using Alkaline And Neutral Comet Assay. The 9th International Conference on Environmental Mutagens, and the 36th Annual Meeting of the Environmental Mutagen Society, SEPTEMBER 3 - 8, 2005, USA (poster).
• Shahidi M, Mozdarani H, Evaluation of radiation-induced DNA damage and DNA repair in prostate cancer patients using neutral single cell gel electrophoresis. 20th Iranian Congress of Radiology.26-29 Jan 2005, Tehran, IRAN (Lecture).
• Shahidi M, Mozdarani H. Study of the radiation sensitivity of leukocytes from healthy individuals and breast cancer patients as measured by the neutral comet assay. 20th Iranian Congress of Radiology.26-29 Jan 2005, Tehran, IRAN (Lecture).
• Shahidi M, Mozdarani H. Comparison of the radioprotective effects of ranitidine and famotidine against gamma-rays as assayed by the single cell gel electrophoresis and micronucleus test. 3rd International WONUC Conference, October 21-23, 2003, Tehran, IRAN (poster).
• Mozdarani H, Shahidi M, A comparison of baseline and induced DNA damage in blood cells from prostate cancer patients and healthy donors using alkaline comet assay. 3rd International WONUC Conference, October 21-23, 2003, Tehran, IRAN (Lecture).
• Shahidi M, Mozdarani H, Garmestani K. A simple procedure to make windowed frosted slides in the laboratory for comet assay. 5th Comet Assay Workshop, August 29-31 2003, Aberdeen, Scotland, ( Invited Speaker).
• Shahidi M., Mozdarani H.; Samiei F. Study of DNA Double and Single Strand Breaks Induced by Gamma Rays Using Comet Assay. 8th Iranian Genetic Conference, May 2003,Tehran, IRAN (poster).
• Shahidi M, Mozdarani H. Potent radioprotective effect of therapeutic doses of ranitidine and famotidine against gamma rays induced micronuclei in mouse bone marrow erythrocytes. Proceedings of international Conference on Role of Free Radicals and Anti-Oxidants in Health & Disease & IInd ANNUAL CONFERENCE OF SFRR_INDIA, FEBRUARY 10-12 2003 ,INDIA (Lecture).
• Shahidi M, Mozdarani H. Effect of ranitidine on Gamma Ray induced genetic effects on bone marrow cells. Proceedings of 14th. Iranian Congress of Physiology and Pharmacology, Tehran University of Medical Sciences. May 1999, Tehran, IRAN. (poster).
• Shahidi M., Mozdarani H., Famotidine reduces the frequency of micronuclei formation in bone marrow erythrocytes. Proceedings of 14th. Iranian Congress of Physiology and Pharmacology, Tehran University of Medical Sciences. May 1999 ,Tehran, IRAN. (poster).
• Shahidi M, Mozdarani H. The protective effects of Ranitidine and Famotidine in preventing genetic aberrations caused by Gamma rays. Proceedings of 9th. International Congress of Geographic Medicine, May 1996, Shiraz, IRAN (poster).

Patents :

• Maryam Shahidi “Electrophoresis tank specially designed for comet assay” Patent Number: 40999, Patent Date: 2007-06-17, issued on Iranian Official Journal, No.18156, Date: 2007-07-01, Page 85.
• Maryam Shahidi “Frosted Laboratory Slides with two clear windows modified for comet assay” Patent Number: 34781, Patent Date: 2006-05-08, issued on Iranian Official Journal, No.17843, Date: 2006-05-31, Page75.
• Maryam Shahidi “A simple device for producing frosted laboratory slides by scientists themselves” Patent Number: 34782, Patent Date: 2006-05-08, issued on Iranian Official Journal, No.17843, Date: 2006-05-31, Page75.